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KMID : 1011620090250020252
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Korean Journal of Food and Cookey Science
2009 Volume.25 No. 2 p.252 ~ p.259
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Quantitative Analysis of Ergosterol as a Biomarker of Mold-contaminated Foods Using the Enzyme Biosensor
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김미경:Kim Mi-Kyeong
김종원:Kim Jong-Won/김미라:Kim Mee-Ra
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Abstract
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Ergosterol is the significant component of the cell wall of fungi. Its presence is regarded as evidence of fungi contamination in grain and other foods. Many studies on ergosterol detection have been carried out using chemical methods, but those methods required complicated pre-treatments and long analysis times. In this study, an amperometric biosensor was developed for fast and precise ergosterol detection. The biosensor system used the electron transfer of hydrogen peroxide produced from the reaction of ergosterol with cholesterol oxidase. The biosensor system consisted of a peristaltic pump, a syringe loading sample injector, an enzyme reactor, a fabricated flow-through cell containing a working electrode, a reference electrode and a counter electrode, and a potentiostat/recorder. The working electrode was prepared by coating modified multi-wall carbon nanotube (MWNT) on glassy carbon electrode. The MWNT-NH2 coated glassy carbon electrode linearly responded to hydrogen peroxide in the range of 1×10-5～8×10-5 M with a detection limit of 10-7 M in the basic performance test. The currents produced from the ergosterol biosensor showed the linearity in a range from 1.0×10-6 M to 1.0×10-5 M ergosterol.
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KEYWORD
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amperometric biosensor, ergosterol, MWNT-NH2, hydrogen peroxide
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